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Western blotting showed that the level of Aβ produced by the L30F/T164A mutant was about 2 times the amount produced by the WT (determine 6b). We used ELISA to quantify Aβforty and also aβ42 generation with the L30F/T164A mutant; we observed that the L30F/T164A mutation resulted in approximately twice as much Aβ40 and Aβforty two, when compared With all the amounts produced by the WT (determine 6c). The ratio of lengthy Aβforty two by using aβ40 was identical in between the WT and mutant (Figure 6d).
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